POST HARVEST THE SCIENCE

ETHYLENE FACT

About Ethylene Gas (C2H4):

Ethylene (C2H4) is an odourless, colourless gas naturally produced and released by fresh fruits and vegetables as a ripening agent.

Ethylene gas is also an ever-present pollutant resulting from exhaust fumes from internal combustion engines (including propane-powered forklifts), improperly vented greenhouse heaters, industrial waste, and similar sources.

Ripening fruit is well known for producing ethylene. However, other plant tissues also produce this gas and can lead to decay and wilting. Even when cut from the growing plant, fruits, vegetables, and flowers are still alive and respiring. Gaseous respiration products of fruits and vegetables, production by normal healthy flower and plant tissue, chopped or damaged leaf tissue, and diseased leaves and tissues can all be significant sources of ethylene.

The Role of Ethylene Gas:

Ethylene molecule and its role in ripening

Ethylene, also known as the “ripening hormone”, plays a regulatory role in many processes of plant growth, development and senescence.

Fruits, vegetables and flowers contain receptors which serve as bonding sites to absorb ethylene molecules. The common practice of placing a kiwi, avocado or banana in a bag to hasten ripening is an example of the action of ethylene on produce: increased levels of ethylene contained within the bag, released by the produce itself, serve as a stimulant to initiate the production of more ethylene. The overall effect is to hasten ripening, ageing and eventually spoilage.

A refrigerator acts in a similar way: while kept closed to retain the desired temperature, it also enables an increased concentration of built-up ethylene. Any closed environment, such as a truck trailer, reefer container, ship’s hold, or warehouse will have a similar effect.

Why Control Ethylene?

The effects of ethylene gas damage are: shrinkage of fresh produce and flower bulbs; spotting of leafy vegetables; yellowing; increased odour and sprouting; wilting of vegetables and cut flowers; scald and loss of crunch; and rind breakdown. Controlling ethylene levels preserves freshness.

Within the industry, it is estimated that losses directly related to ethylene run into billions of dollars annually. Removal of ethylene from the storage and shipping environment delays spoilage, reduces loss and increases profit.

Ethylene will penetrate most substances. It will permeate through produce cardboard shipping boxes, wood and even concrete walls.

Ethylene is harmful to many fruits, vegetables, and floral items. While ethylene is invaluable due to its ability to initiate the ripening process in several fruits, it can also be very harmful to many fruits, vegetables, flowers, and plants by accelerating the ageing process and decreasing product quality and shelf life. The degree of damage depends upon the concentration of ethylene, the length of exposure, and product temperature. One of the following methods should be used to ensure that ethylene-sensitive produce is not exposed:

  1. Ethylene-producing items (such as apples, avocados, bananas, melons, peaches, pears and tomatoes) should be stored separately from ethylene-sensitive ones (broccoli, cabbage, cauliflower, leafy greens, lettuce and similar). Ethylene is also emitted by engines — propane, diesel and petrol powered engines all produce it in amounts large enough to damage the ethylene-sensitive produce mentioned.
  2. Ventilate the storage area, preferably to the outside of the warehouse, on a continuous or regular basis to purge the air of any ethylene.
  3. Remove ethylene with ethylene absorbing filters. These have been proven in reducing and maintaining low ethylene levels. If ethylene damage is suspected, a quick and easy way to detect ethylene levels is with hand held sensor tubes. This will indicate whether the above steps should be followed.

Ethylene has been scientifically proven to cause:

  • Increase in respiration
  • Water loss and weight loss
  • Yellowing or spotting
  • Production of more ethylene
  • Accelerated ripening, ageing and decay
  • Reduction in nutrient content, for example loss of vitamin C
  • Taste and aroma changes, off-flavours such as bitterness
  • Loss of leaves and flowers, sprouting, toughening

FURTHER READING

The research these pages draw on is published in subscription journals and industry magazines. Rather than host copies, we have summarised the findings — what each study measured, what it concluded, and what it means in a packing shed — with links to the original sources.

  1. Postharvest ethylene research: what the studies actually found — three Australian studies on ethylene thresholds, measured levels in real supply chains, and how much shelf life is lost. Wills et al. (1999, 2001) and Warton et al. (2000), Australian Journal of Experimental Agriculture.
  2. Ethylene management in postharvest handling — climacteric and non-climacteric crop tables, crop-specific damage symptoms, controlling sources in the shed, and removal methods. Drawn from Sydney Postharvest Laboratory information sheets.
  3. How ethylene absorption works: the science behind KMnO4 scavengers — the oxidation chemistry, and what determines how well a scavenger performs.
  4. Postharvest fundamentals: temperature, moisture and the cost of delay — field heat, pre-cooling, why refrigeration itself dries produce out, and cool room air circulation. Drawn from Sydney Postharvest Laboratory information sheets.

Ethylene in your cold room or container?

Biopac supplies KMnO4 ethylene absorption in two formats — filters for the storage room or reefer, and Fresh Up® sachets for inside the carton. Which one you need depends on the crop, the load and the length of the chain.

See ethylene filters
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Modified Atmosphere Packaging for Fresh Produce

Modified atmosphere packaging for fresh produce

Modified Atmosphere Packaging uses the gases produced and consumed during the respiration of fresh produce — carbon dioxide (CO2) and oxygen (O2) — to create a favourable atmosphere inside a specially designed polymeric film package. A unique atmosphere for that product is created, enriched in CO2 and reduced in O2. This slows the metabolic activity of the produce to a very low level, enabling highly perishable produce to be stored for prolonged periods.

The ability to store produce while retaining its food and nutritional value increases market flexibility.

Growers and packers can add value by storing during market gluts and releasing when demand increases, and by branding and differentiating produce as high quality and high value.

Exporters can use relatively cheap and available sea freight rather than air freight, which increases competitiveness in existing markets and opens access to new ones.

How Respiration Drives the Package

All fresh produce is alive and must burn food reserves to stay alive. The chemical reactions involved are generally termed respiration. In normal healthy plants, respiration involves the uptake of oxygen (O2) by the plant tissue to oxidise food reserves, usually sugars, producing energy, carbon dioxide (CO2) and water. The energy maintains essential life processes, while the CO2 and some of the water are waste products released to the surrounding environment.

When fresh produce is sealed inside a MAP bag, respiration lowers the in-package O2 level and raises the CO2 level. The central challenge in designing a modified atmosphere package is matching the rate of O2 uptake and CO2 production of the produce to the O2 and CO2 permeability of the film. Get that match right and gas levels inside the package equilibrate within a range that benefits the produce.

Optimum CO2 and O2 concentrations are product specific and vary enormously between products. Exact recommendations are rare, and a therapeutic range is more commonly quoted, since optimum gas levels vary according to cultivar or genotype, production area, harvest maturity and several other factors.

What the Right Atmosphere Achieves

When the optimum atmosphere for a product is achieved, its storage life can be increased many times over what conventional refrigerated air storage delivers. Elevated CO2 and reduced O2 levels slow quality loss in several ways, and the principal effect is usually considered to be suppression of respiratory activity.

The postharvest life of fresh horticultural produce is inversely proportional to its respiration rate. The lower the respiration rate, the longer food reserves are conserved and the longer life processes can be maintained. This effect is often described as putting the produce to sleep. There are several further benefits — high CO2 and low O2 atmospheres can:

  1. Block the mode of action and biosynthesis of ethylene, the plant hormone that promotes ageing and senescence.
  2. Reduce rots by directly inhibiting the growth of pathogens, and by maintaining the health and integrity of the plant tissue, which reduces its susceptibility to infection.
  3. Slow yellowing of green tissues by preventing chlorophyll degradation.
  4. Maintain food value, nutritional value and flavour by slowing the loss of food reserves, particularly sugars, inhibiting the loss of labile vitamins such as C and A, and slowing the accumulation of undesirable secondary metabolites such as free ammonia.
  5. Slow cell membrane degradation and the loss of cellular compartmentation and function.
  6. Inhibit discoloration of cut surfaces.

Why It Matters Commercially

MA and MH packaging deliver significant benefits to fresh produce by reducing quality loss and extending storage and market life. For the horticultural industry it is a means of adding value to a crop by expanding existing markets and opening new domestic and export ones.

The commercial case has never been stronger. Hort Innovation’s 2024/25 Australian Horticulture Statistics Handbook records total industry production value at a record $18.4 billion, up 6% on the prior year, with fruit reaching $7.3 billion and vegetables $6 billion — both records.

Exports are where the growth is concentrated. Total fresh export value reached a record $3.5 billion in 2024/25, up 13% in a single year and more than double what it was a decade ago. Over that decade exports grew at around 8.5% a year against 3.5% for the domestic wholesale market — more than twice the rate.

That growth is concentrated in exactly the markets where shelf life determines whether a shipment is viable. Exports to China rose 58% in the year, with further increases into India, Vietnam, Hong Kong, Japan and Indonesia. Bananas and melons both reached record values.

Distance is the constraint. Every one of those destinations is far enough away that the choice between air freight and sea freight decides whether the margin survives the journey — and that choice depends entirely on whether the produce can hold condition long enough to arrive in specification.

Source: Hort Innovation and Kynetec, Australian Horticulture Statistics Handbook 2024/25. View the handbook

Related Reading

Need a MAP bag engineered for your crop?

Every Lifepack® MAP bag is specified to the respiration rate of a particular crop and carton — OTR, CTR and WVTR matched to what the produce actually does. Tell us the crop, the pack size and the destination.

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